It's the last day of the quarter. Thank god for that.
Things haven't been going well recently with the cloning. We've been having difficulty in most of the steps so far, sans the step where we introduce the insert into the bacterial plasmid. Plates failed to grow when all indications showed they would thrive, PCRs have turned up negative despite huge amounts of DNA and now someone transferred our plates to the wrong incubator without telling us, shaving a day off.
Now, one last assay has emerged on the horizon. How much of each kind of bacteria are there in the soil? After we standardize the DNA concentration between the different regions, we can finally get an estimate of just how different things are in the varying locations along the river. Using PCR, we will use all 8 different primer sets, and enhance the present DNA to a point where we can show the abundance of difference families of bacteria well.
Ok. Back to spanish. Big test tomorrow morning.
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