So, as of now we haven't gotten very far at all into the cloning process...the first steps of mixing the DNA and plasmid and getting them into the bacteria worked, but only just. When we went to plate our solutions out to pick the white colonies from the blue colonies, we got bad results, which either manifested as a lawn of bacteria, or a small amount of blue and white colonies, in tight, hard-to-work-with groupings. According to Bill, however, everything we were doing seemed right on the money, so he's currently incubating another batch so we can look at them this coming tuesday.
Hopefully, it will give us lots (50+) distinct colonies per plate, and not an unusable lawn or 'hot spots'. Once that is over we can pick and patch and grow each colony properly by itself to isolate the certain DNA sequence.
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