Finally, the work I've been waiting for all year has come! PCR, Gels, cloning and DGGE. No more nasty soil samples to work with or mindless stick-soil-in-tube-wait-half-hour protocols. By friday evening, 6 more PCRs and their respective gels will be complete, followed closely by Denaturing Gradient Gel Electrophoresis and some cloning into E. coli bacteria.
The DGGE and cloning are going to be the ways that we can discover exactly what is in the soil. For now, there's just six tubes filled with a LOT of different bacterial DNA, and no way to draw any conclusions about their respective regions. By isolating and splicing the DNA into a bacterial genome, we can create a clone colony which represents the DNA's former owner.
There'll be more details in the next post
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